FastQCFastQC Report
Sun 14 Apr 2019
SRR6575948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520058
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8910.17132704429121368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.12652434920720382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGA250.00523585356.3961337
GTATCAA13300.045.7328381
TCAACGC17100.035.7354164
AACGCAG17150.035.083066
ATCAACG17350.034.681983
CAACGCA17500.034.6500055
GTACATG29300.033.6332441
TACATGG29450.032.8832932
ACATGGG29800.032.1815723
ACGCAGA20300.029.6334367
TATCAAC20550.029.510142
CGCAGAG21100.028.7326288
CATGGGA14950.027.6689764
GTAATAG1406.3448533E-726.863053
ATGGGAG7400.026.0437645
GCAGAGT24400.024.8466579
GGTATCA6850.024.0917051
CATGGGC6050.023.3086684
CATGGGG12650.023.0384254
AGTACAT19150.022.5846022
Produced by FastQC (version 0.11.2)