FastQCFastQC Report
Sun 14 Apr 2019
SRR6575949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072395
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13110.12224973074286993No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12670.11814676495134721No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12080.1126450608218054No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12020.11208556548659776No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11970.11161931937392472No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11330.10565136913170986No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11310.10546487068664066No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11250.104905375351433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.058.6110081
GTATCAA16250.057.442711
TCAACGC23250.039.6295244
ATCAACG23600.038.6434143
CAACGCA25050.036.5925255
AACGCAG25500.036.131126
GTACTAG851.71841E-533.2778631
TATCAAC29200.032.0373042
GTACATG41650.030.334921
TACATGG41450.029.7140962
ACATGGG41800.029.1279053
ACGCAGA31550.028.8979237
CGCAGAG33850.027.0732368
CTATTAA3050.026.2767831
GCAGAGT35750.025.7658399
AGAGTAC31700.024.98071710-11
CATGGGG24500.024.3681964
ACCTAAG4800.023.5718211
TATTAAG3700.022.8694932
TAGGTAT2905.456968E-1222.693195
Produced by FastQC (version 0.11.2)