FastQCFastQC Report
Sun 14 Apr 2019
SRR6575950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942370
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11950.12680794167895837No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11590.12298778611373451No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11210.11895539968377603No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11050.11725755276589873No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10830.11492301325381751No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10580.11227012744463427No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9970.1057970860702272No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9820.10420535458471726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15100.066.726311
ATCAACG20900.046.9926573
GGTATCA4650.046.5763171
TCAACGC21150.046.4371834
CAACGCA21850.044.734425
AACGCAG22600.043.4578066
TATCAAC26350.038.164812
ACGCAGA28950.033.7632877
CGCAGAG29800.032.822888
GTACATG37550.030.7196621
GCAGAGT32750.029.8663169
ACATGGG38550.029.1342263
TACATGG39550.028.9916742
GTCTTAG5100.025.8492621
AGAGTAC29300.025.2779910-11
CATGGGG23200.024.3065434
TAACACT1551.5483783E-624.2542724
TAGGTAT3350.023.8470155
TATAGGG1201.8396525E-423.4963252
GTATATA2052.9298462E-822.9671131
Produced by FastQC (version 0.11.2)