FastQCFastQC Report
Sun 14 Apr 2019
SRR6575951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730652
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16960.23212144769329313No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13150.17997624039898613No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9960.13631660489535374No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG8440.1155132676020869No Hit
TCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT7770.1063433755057127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.10593278332229296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16750.063.702341
GGTATCA4800.048.9635731
TCAACGC23200.045.7831154
ATCAACG23650.045.1107033
CAACGCA24000.044.452845
AACGCAG24250.043.9945646
TATCAAC24850.043.127352
ACGCAGA30050.035.50317
CGCAGAG30050.035.50318
GCAGAGT33300.032.0380829
GTACATG39350.031.655231
ACATGGG40900.030.106683
ATACCGT1751.70985E-1029.5419736
TACATGG43150.029.0853822
ACCAGAT7450.028.38841494
CATGGGA19950.027.0919234
TATAGGG1057.4125375E-526.8600162
TACCGTC1755.5479177E-926.856347
CTTAGGT3200.026.4367123
AGAGTAC31100.026.14391510-11
Produced by FastQC (version 0.11.2)