FastQCFastQC Report
Sun 14 Apr 2019
SRR6575952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662621
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14580.22003528412169246No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11750.17732610345883998No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9270.1398989769415699No Hit
TCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT7590.11454511704277408No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7510.11333779038092665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.10292459792249264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15650.065.179591
GGTATCA4750.062.3466571
CTATATG405.6705653E-447.0073971
TCAACGC23200.042.9355854
CGGGCGT555.2898777E-542.714656
CAACGCA23500.042.3874745
ATCAACG23700.042.228033
TATCAAC24300.041.587772
AACGCAG24600.040.8741046
AATATCG500.001703977837.5888945
ACGCAGA29600.033.8109557
CGCAGAG29750.033.640488
GCAGAGT32950.030.516029
GGTAATC1701.2187229E-1030.402788
GTACATG37550.029.543931
TACATGG38550.028.5315192
ACATGGG38200.028.290073
CTTAGGT3350.028.0514133
AAGGGTA1853.2923708E-1027.937695
CATGGGG19250.027.3373784
Produced by FastQC (version 0.11.2)