FastQCFastQC Report
Sun 14 Apr 2019
SRR6575952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662621
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16800.25353859898795844No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11890.17943892511707296No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9270.1398989769415699No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG8380.12646746782851737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.10624474624257305No Hit
TCCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGT6800.10262276625703079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.064.1049961
GGTATCA4250.057.557551
TCAACGC21700.042.6614234
ATCAACG22150.041.7947123
TATCAAC23250.040.628872
CAACGCA22700.040.5750475
AACGCAG23100.039.8724486
ACACCCG500.001695274737.62809894
ACGCAGA28500.032.317677
CGCAGAG28750.031.8972638
ACCTAAG6100.031.6185471
TGTACGT600.00416142131.3307192
CGTGTAC600.00416295931.3283523
GTACATG38450.029.4855041
CTAAGAC6900.029.28523
GCAGAGT31500.029.2618759
TAAGACA8550.029.1298734
ACATGGG38900.028.5096043
TACATGG39800.028.221262
ATAGGGT1853.274181E-1027.9415043
Produced by FastQC (version 0.11.2)