FastQCFastQC Report
Sun 14 Apr 2019
SRR6575954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320908
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5730.1785558477819188No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA4850.15113365824473057No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4590.14303164769965224No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4570.1424084161192616No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4510.14053872137808968No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC4440.13835741084672243No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT4340.1352412529447692No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4260.13274832662320665No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT4210.13119024767223006No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG3890.12121854238597979No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3720.11592107395265933No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3690.11498622658207337No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3680.11467461079187805No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3600.11218168447031547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.11031198972914356No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3490.10875391077816694No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3440.10719583182719035No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG3360.10470290550562777No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC3240.10096351602328395No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3230.1006519002330886No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA3220.10034028444289328No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3210.10002866865269798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.067.3827061
TATCGGG250.005216319656.44444394
CAACGCA11500.045.767165
ATCAACG11550.045.5690353
GGTATCA2500.043.2875751
TATCAAC13150.040.3818742
TCTAACG1051.1350494E-940.279772
AACGCAG13300.039.926446
ACGCAGA13750.037.936227
CGCAGAG13800.037.8223428
TCAACGC13950.037.72924
GTACAGA1152.732122E-936.8230821
CTAACGC1152.7666829E-936.7771843
GCAGAGT14600.035.7498869
ATATAAC1107.676863E-834.1767773
TAGTACT1850.033.0221524
CGCCTAC1309.156793E-932.533667
GTACTAG1501.0513759E-931.3678131
ATAAGGG600.004159556231.328713
ACGCCTA1401.884655E-830.2098276
Produced by FastQC (version 0.11.2)