FastQCFastQC Report
Sun 14 Apr 2019
SRR6575957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606147
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9000.14847883434216452No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8720.1438594928292972No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8590.14171479855546593No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8510.14039498669464667No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8320.13726043352520098No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8120.1339609038731529No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7900.13033142125589997No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7410.12224757360838212No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7120.11746325561291238No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6940.1144936789260691No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6890.11366879651305707No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6880.11350382003045464No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6750.11135912575662339No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6750.11135912575662339No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6680.11020429037840655No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6590.10871950203498491No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6430.10607987831334642No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6370.10509001941773201No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6220.10261537217869593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.053.9371531
CTTAGGT751.4801117E-743.864133
ATCAACG16900.040.0450173
CAACGCA16900.039.488845
TTAGGTA1003.324203E-837.597824
AACGCAG18650.036.5394366
TCAACGC19900.034.008084
TAGGCGG1256.2609615E-933.838045
GGTATCA5350.033.3922651
GTACTAG2400.033.3007281
ACCTAAG1309.147698E-932.5473181
GTACATG26650.032.459111
TACATGG26250.032.4164352
TCTTAGG1606.002665E-1132.3212972
CTATTGA4100.032.0957039
ACATGGG26400.031.6875573
CTTAAAC1052.0641692E-631.3315183
TAGGTAT1052.0641692E-631.3315185
TATCAAC22700.031.2728272
ACGCAGA21150.031.1093087
Produced by FastQC (version 0.11.2)