FastQCFastQC Report
Sun 14 Apr 2019
SRR6575957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606147
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8480.13990005724683946No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8290.13676550407739377No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8190.13511573925136972No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7830.12917658587768313No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7730.12752682105165908No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7510.12389733843440617No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7290.12026785581715327No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7240.11944297340414124No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7150.11795818506071959No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6650.10970936093059934No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6620.10921443148279213No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6410.10574992534814161No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6360.1049250429351296No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6310.10410016052211757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.053.3548161
TAATAGT503.005593E-547.0056534
CAACGCA17800.038.55525
ATCAACG18100.038.435563
AACGCAG20450.034.708336
TCAACGC21150.033.559594
ACGCAGA22000.031.1895127
CGCAGAG22200.030.9085278
TATCAAC23200.030.5941942
ATTAACT2650.030.1545684
GTACATG26700.028.9488181
TATTCTC2600.028.9265565
ACATGGG26450.028.7898523
GTACTAG3300.028.563691
GTATAAG3300.028.563691
GCAGAGT24000.028.3945629
TACATGG27300.028.0656452
AGAGTAC22000.027.34422910-11
ATACTGT1555.0691597E-827.2936046
CCACTAA700.0087145126.931481
Produced by FastQC (version 0.11.2)