FastQCFastQC Report
Sun 14 Apr 2019
SRR6575960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623296
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10260.1646087894034295No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8500.13637180408666189No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7930.1272268713420269No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7800.12514118492658385No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7760.12449943526029365No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7390.11856325084710956No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7070.11342925351678816No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7060.11326881610021562No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6820.10941831810247458No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6660.1068513194373139No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6630.10637000718759626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.10556782010473355No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6410.1028403840230003No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6340.10171732210699251No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6340.10171732210699251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.059.6929361
ATCAACG20500.040.5773353
CAACGCA20450.040.4467355
AACGCAG23350.036.027186
TCAACGC23250.035.3735964
AGGGCTA4300.034.9740265
ACGCAGA23800.034.3586737
TATCAAC24550.034.2661742
CGCAGAG24450.033.637478
GTATAAG4800.031.4292341
CTATTGA4400.030.9748699
ACATGGG28000.030.7154713
ACCTAAG2000.030.6435051
GTACATG28000.030.4751341
GCAGAGT26650.030.3316029
GGCTATT4650.030.3202257
TACATGG28400.030.1173782
GGGCTAT4550.029.9537166
GGTATCA4900.029.8257031
GAACGAT650.00606085629.01161
Produced by FastQC (version 0.11.2)