FastQCFastQC Report
Sun 14 Apr 2019
SRR6575961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570628
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10410.18243058524993516No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7810.1368667503171944No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7010.12284710879942799No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6910.1210946536097072No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6760.118465970825126No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6680.11706400667334936No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6500.11390958733185191No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6490.11373434181287984No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6480.11355909629390776No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6410.11233237766110321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.10970369487652201No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6240.10935320383857784No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6140.10760074864885705No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6060.10619878449708042No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6020.1054978024211921No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6020.1054978024211921No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5910.10357010171249921No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5880.10304436515558298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.059.4395261
ATCAACG20500.040.1162643
CAACGCA20950.039.254585
ACCTAAG1551.8189894E-1236.452221
TATCAAC23250.035.97772
AACGCAG23250.035.9745456
TCAACGC23500.035.3949854
CGTTAAC1651.8189894E-1234.2429921
AGGTATA1256.2700565E-933.832246
ACGCAGA24650.033.5530557
CGCAGAG24850.033.4926648
CTTAGGT1606.002665E-1132.3079223
CTATTAT1501.0477379E-931.389411
TCTATAC753.2762115E-431.3288943
GCAGAGT26950.030.7083669
GGTATCA4800.030.4084951
ACGCAAG804.7818664E-429.3708387
TAGGTAT1452.6588168E-829.1682785
ATAGAGT650.00615850228.9164456
ACATGGG26950.028.4226913
Produced by FastQC (version 0.11.2)