FastQCFastQC Report
Sun 14 Apr 2019
SRR6575962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818047
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12550.15341416813459374No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11650.1424123552803201No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10560.12908793749014422No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10420.12737654437947943No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10390.12700981728433697No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10210.12480945471348225No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10050.12285357687272247No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9780.11955303301644038No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9300.11368539949416111No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9280.11344091476406612No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9240.1129519453038762No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9240.1129519453038762No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9150.11185176401844882No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8510.10402825265540977No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8440.10317255610007739No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8300.10146116298941259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17800.053.4849321
ATCAACG23950.039.450043
CAACGCA24050.039.0857735
GGTATCA7750.036.487961
AACGCAG27700.035.29296
TATATAC550.00271474334.1864623
TCAACGC29300.032.2427644
ACGCAGA29450.031.916997
TATCAAC31250.031.7387142
CGTTAAC2100.031.420191
GTACTAG4300.030.689491
TAACAGT2150.030.605074
CGCAGAG30850.030.468578
GGGTACC4200.030.2128527
CGTGTAC650.006149061528.9270083
GGTACCT4400.028.8395398
GTATAAG5400.028.8018421
GCAGAGT33200.028.4534689
GTACATG35000.028.4128281
TACATGG35000.028.2038332
Produced by FastQC (version 0.11.2)