FastQCFastQC Report
Sun 14 Apr 2019
SRR6575963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761281
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11130.1462009428844277No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10730.1409466412533611No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9800.12873038996113131No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9760.12820495979802465No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9600.126103239145598No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9550.1254464514417147No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9270.12176844029996808No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9240.12137436767763808No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9190.12071757997375476No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9160.12032350735142476No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8630.11336155769026154No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8590.11283612752715488No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8400.11034033425239827No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8320.10928947392618495No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8250.10836997114074828No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8210.10784454097764162No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8120.10666232311065166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18950.053.5674671
GCATATA1051.1386874E-940.2818831
CAACGCA25350.039.8502925
ATCAACG25900.039.0040473
AACGCAG29000.035.6447566
GTTAGGC801.1604561E-535.2397043
ACGCAGA29200.034.5960547
TCAACGC29850.034.1575244
CGCAGAG29800.033.899498
TATCAAC31150.033.04022
GGTATCA8650.032.5980571
GACGTTA600.00416605831.3241837
CAGTACG902.5979634E-531.3241839
GCAGAGT32450.031.131129
TAGGTAT1452.6651833E-829.1638935
AGAGTAC30850.027.56731210-11
CTACGCT700.00884677426.8492984
ACTACGC700.00884677426.8492983
AGTACTT11250.026.31231512-13
TACATGG33600.026.295122
Produced by FastQC (version 0.11.2)