FastQCFastQC Report
Sun 14 Apr 2019
SRR6575963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761281
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11340.14895945124073764No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10260.13477283683685787No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9850.12938717766501465No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9610.12623459668637468No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9470.12439559111550137No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9420.12373880341161804No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9330.12255658554462807No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9300.12216251292229807No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9200.12084893751453142No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9170.12045486489220143No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8940.11743364145433814No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8840.11612006604657149No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8350.10968354654851493No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8310.10915811638540827No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8190.10758182589608829No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7910.10390381475434168No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7760.10193345164269173No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7740.1016707365611384No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7660.10061987623492508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.055.2880941
GGTATCA6850.040.5610961
ATCAACG22900.039.400363
CAACGCA22800.039.3670585
AACGCAG26100.035.2898676
TCAACGC27250.033.110764
CGCAGAG27150.032.9081578
ACGCAGA27350.032.815727
TATCAAC29650.031.8570672
CGTTAAC1701.200533E-1030.4713731
GTACATG33100.030.3039931
GGTTTAA1401.8535502E-830.2735061
GCAGAGT29900.029.8814879
TACATGG33600.029.510572
ACATGGG33550.028.9942743
ACCTAAG2300.028.6647681
CATGGGG22050.028.5581874
ATAGTAC1057.4257136E-526.853223
ATGGGGG11900.026.458325
TAGGTAT909.522829E-426.1072965
Produced by FastQC (version 0.11.2)