FastQCFastQC Report
Sun 14 Apr 2019
SRR6575965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998575
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12960.129784943544551No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12770.12788223218085773No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12220.1223743834964825No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11740.11756753373557319No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11020.11035725909420925No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10250.10264627093608393No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10210.10224570012267481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16550.058.798371
GGTATCA5700.051.9587481
TCAACGC23350.041.050534
ATCAACG24250.039.3332443
CAACGCA25050.038.6398055
AACGCAG25250.038.333756
TATCAAC29000.033.5556262
CGCAGAG31850.030.3901798
ACGCAGA32050.030.2005377
ACTACGC804.7883322E-429.3667223
TTACGCA650.006159250628.91782494
ATAGGGT856.826694E-427.6392693
GTACATG41000.027.6328811
GCAGAGT36100.027.07279
GATATAA1057.409558E-526.8630281
ACATGGG42000.026.6258283
TACATGG42100.026.5758912
ATAAGGG1258.04011E-626.3125843
ACCTAAG5400.026.1168291
TACACCG1659.3405106E-825.6291395
Produced by FastQC (version 0.11.2)