FastQCFastQC Report
Sun 14 Apr 2019
SRR6575965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998575
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12800.12818266029091455No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12460.12477780837693714No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12060.12077210024284606No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11190.11205968505119795No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11180.11195954234784569No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11060.11075782990761837No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10400.10414841148636807No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10260.10274641363943621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.060.3619581
GGTATCA5300.054.189091
TCAACGC23550.043.7002534
ATCAACG24150.042.809123
CAACGCA24600.041.8350035
AACGCAG25900.039.916616
TATCAAC30500.034.6737172
ACGCAGA32050.032.5503547
CGCAGAG32350.032.2727368
GCAGAGT35700.029.112619
GTACATG39250.028.7891921
TACATGG41500.026.9554522
ACATGGG41950.026.4369183
AGAGTAC32050.025.82508310-11
GTCTTAG4800.025.5029261
GTATAAG2202.401066E-923.5411621
AGGTATA3050.023.1111856
TAGGTAT3300.022.7843595
CTTAGGT3100.022.738423
CATGGGG26000.022.4119244
Produced by FastQC (version 0.11.2)