FastQCFastQC Report
Sun 14 Apr 2019
SRR6575968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114980
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13400.12018152791978332No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13110.11758058440510144No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12780.11462089006080826No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12660.11354463757197437No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11170.1001811691689537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.061.732311
GGTATCA5850.060.2711871
TCAACGC27450.044.173814
ATCAACG27800.043.6176643
CAACGCA28850.042.19315
AACGCAG30150.040.373836
TAGCGCA608.8251516E-539.1657334
TATCAAC33800.035.6063652
ACGCAGA37600.032.374237
CGCAGAG39200.031.4125168
GCAGAGT41600.029.7132329
GTACTAG1154.1882377E-628.6157131
GTACATG48700.028.3806761
TACATGG49600.027.5813622
ACATGGG49650.027.546173
ACCTAAG5350.025.4828851
AGAGTAC37950.025.202310-11
CTAAGAC6800.024.1906013
CATGGGG29150.023.862214
TAAGACA6600.022.7873364
Produced by FastQC (version 0.11.2)