FastQCFastQC Report
Sun 14 Apr 2019
SRR6575969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011344
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12660.12517995855020647No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12110.11974165071429702No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11590.11459997785125536No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10670.1055031720166432No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10370.10253682228796532No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10300.10184467401794048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16600.064.011051
GGTATCA4850.062.0430151
ATCAACG24050.043.3723833
TCAACGC24150.043.1927874
CAACGCA25100.041.5580025
AACGCAG26350.039.94326
TATCAAC28700.036.5305442
ACGCAGA32850.031.8966377
CGCAGAG35000.029.8030228
GTACATG42000.029.1057421
GTATAAG2300.028.6190241
TACATGG42900.028.0553572
GCAGAGT37750.027.6319439
ACATGGG43500.027.3279273
TGATCGC909.5325586E-426.1037489
AGGTATA2701.8189894E-1224.36356
AGAGTAC33700.024.19050610-11
GGGTACC7450.023.9663967
TAGGTAT2751.8189894E-1223.9205285
TTAGGTA2950.023.8915674
Produced by FastQC (version 0.11.2)