FastQCFastQC Report
Sun 14 Apr 2019
SRR6575970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378344
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6740.17814475715222125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.11841075846319753No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4370.11550335144736008No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4110.10863129850083521No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4070.10757405958598525No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3830.10123062609688536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.033.6801641
TACATGG27000.032.3859752
GTACATG26900.032.156841
ACATGGG27200.031.9665533
ATGGGAG4600.029.6301025
GGTCGTT650.0061493628.922757
AACGCAG15900.028.0814486
CATGGGG9550.027.5599024
CATGGGA14550.027.4567384
TAATGGT1904.456524E-1027.210224
TAGGGTG700.00882915126.856845
CAACGCA16600.026.6141595
TCAACGC16350.026.4461844
GTATTAC909.4918E-426.1177221
ATTATGC909.5064624E-426.1108173
ATCAACG16650.025.9696793
TATCAAC18450.024.7162842
ATTATCC1151.3749293E-424.5214633
CGCAGAG18500.024.1348638
CATGGGT5950.023.6972124
Produced by FastQC (version 0.11.2)