FastQCFastQC Report
Sun 14 Apr 2019
SRR6575970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378344
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6910.18263802254033368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.1424629437760345No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4920.13004038652654726No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4480.11841075846319753No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4070.10757405958598525No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4020.10625251094242276No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3820.10096631636817288No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3790.1001733871820354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.036.8328361
CATGGGG8500.034.2810024
ACATGGG24700.033.869113
GTACATG24900.033.0920641
TACATGG25650.032.7979282
AACGCAG16000.028.198896
CAACGCA16250.027.765065
ATCAACG16300.027.6798923
TCAACGC16400.027.5111124
ATGGGGA4700.026.9989385
GTACTAT700.00875094626.9058721
AGAACCG1406.351529E-726.8560855
CTAGGTC700.00883036126.85608594
GTATAAC909.4057596E-426.1584851
ATGGGAT3100.025.7731785
CATGGGA13250.025.1839144
CGCAGAG18500.024.6422738
ACGCAGA18550.024.5758537
GTATATA2501.6370905E-1124.4843431
CATGGGT5600.024.3383274
Produced by FastQC (version 0.11.2)