FastQCFastQC Report
Sun 14 Apr 2019
SRR6575972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125063
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15690.1394588569706763No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15620.13883666959094734No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14340.12745952893304643No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14050.12488189550274073No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13520.12017104819907862No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13130.11670457565487445No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13030.11581573654097592No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12390.11012716621202547No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12280.1091494431867371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14850.062.67121
GGTATCA4400.057.685991
TCAACGC23100.040.2743034
CAACGCA23650.039.3376925
ATCAACG23650.039.3376923
AACGCAG24900.037.7403146
TACATGG44250.030.804492
GTACATG44800.030.7410641
TATCAAC31100.030.2272682
ACATGGG45450.029.670363
ACGCAGA31700.029.6445967
GGTACCT7500.028.8185048
CGCAGAG33150.028.206198
CTAAGAC4650.026.2721253
GGGTACC8250.026.1986397
ATGGGTA9350.026.1316345
ACCTAAG2700.026.1131
GCAGAGT38000.024.6061889
AGAGTAC32850.024.38730810-11
CATGGGG26350.023.894564