FastQCFastQC Report
Sun 14 Apr 2019
SRR6575972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125063
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15120.1343924740214548No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14570.12950385889501298No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13990.12434859203440164No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13240.11768229868016279No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12980.11537131698402667No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12350.10977163056646605No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA12170.10817172016144873No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11450.10177207854137946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17250.062.757511
GGTATCA5300.047.0681341
TCAACGC23300.045.3793334
ATCAACG23700.044.6134383
CAACGCA23950.044.343965
AACGCAG24900.042.463396
ACGCAGA29950.035.303467
TATCAAC31700.034.2470252
CGCAGAG33450.031.6306088
GCAGAGT37050.028.9379549
GTACATG41900.027.6342741
TACATGG42200.027.173622
ACATGGG42450.026.7897833
GGGTACC7750.026.6797967
GGTACCT7450.025.2478778
AGAGTAC32450.024.92498410-11
TAGTACA2052.9909643E-822.9233264
GTACCGT1050.002337537722.3775356
GTATATA1903.5891935E-722.2954311
GTATACA3007.2759576E-1221.9651281