FastQCFastQC Report
Sun 14 Apr 2019
SRR6575973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173345
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2370.13672156681761805No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2250.1297989558395108No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT2040.11768438662782313No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1920.1107617756497159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAT156.8949343E-493.990763
GTATCAA2150.074.404151
TCAACGC2650.063.842794
ATCAACG2800.060.4226383
CAACGCA2900.058.3390965
AACGCAG2950.057.3502966
ACGCAGA3550.047.6572887
TATCAAC3500.045.7054062
CGCAGAG3800.044.5219428
TACATGG6250.040.6509252
GTACATG6400.040.4333231
ACATGGG6600.037.738723
GCAGAGT4600.036.7789969
CATGGGA2800.031.8897254
ATGGGAG1351.3147655E-831.330265
CATGGGG4100.030.9481814
ATTATAC650.00614290228.9202353
ATACACT650.00614290228.9202354
AGAGTAC3850.028.68549510-11
GTATATC350.00883189126.85450634-35
Produced by FastQC (version 0.11.2)