FastQCFastQC Report
Sun 14 Apr 2019
SRR6575973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173345
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2230.1286451873431596No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1790.10326228042343304No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC1740.10037785918255503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG205.672489E-446.99783314-15
GGTATCA1201.8189894E-1243.181021
GTATCAA2250.041.87251
ATATAAC500.001698426937.5982673
ATTATCC651.4089249E-436.1521843
GTACATG5900.031.9366551
ATACTGT753.2650464E-431.3318926
ACCAAGC753.2650464E-431.3318927
TCAACGC3200.030.8423274
ACATGGG5850.030.5285113
ATCAACG3250.030.3678343
AACGCAG3250.030.3678346
ACCTAAG953.6768026E-529.7515141
TACATGG6150.029.039312
CATGGGG3650.028.3274614
CAACGCA3500.028.1987025
TAGGTAA856.794421E-427.6457845
CTTATTT700.00881768626.8559073
CTACCTA350.00882964626.85590732-33
GTCTTAG1109.979624E-525.694491
Produced by FastQC (version 0.11.2)