FastQCFastQC Report
Sun 14 Apr 2019
SRR6575974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156714
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2290.14612606404022616No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2000.12762101662901845No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1740.11103028446724607No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT1670.10656354888523042No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1580.10082060313692459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1950.057.84911
GGTATCA851.8189894E-1255.2969321
CTAGGAC450.001012609641.7665753
CCTTATT500.001697119237.6019172
TCAACGC3000.037.589924
ATCAACG3100.036.3773423
TAGGTAT651.41015E-436.144155
GTACATG5500.035.892741
ACATGGG5800.034.0253563
CAACGCA3350.033.6626135
GTCTTAG851.7445615E-533.1781581
TACATGG6050.032.629762
TCATAAA600.00414908531.3349322
CTCTACG600.00414908531.3349321
AGGTATA753.2678826E-431.3249346
TAATACT600.004155574431.324934
TAGGACA600.004155574431.324934
CTTAGGT600.004155574431.324933
AACGCAG3700.030.4783126
TATCAAC3900.030.129742
Produced by FastQC (version 0.11.2)