FastQCFastQC Report
Sun 14 Apr 2019
SRR6575979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486800
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10670.21918652423993426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.15427280197206245No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7280.1495480690221857No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7040.14461791290057519No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5040.10353327855382086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.071.291541
GGTATCA3400.055.2982251
ATCAACG22250.042.245993
TCAACGC22400.041.7532774
CAACGCA22900.040.8416335
TATCAAC23250.040.6352772
AACGCAG23700.039.661326
TATAGGG801.1566903E-535.2526172
GTACATG35500.034.027881
ACGCAGA27750.033.703557
ACATGGG35750.032.997663
CGCAGAG28400.032.6011858
TACATGG37800.032.081752
CCTTTAT1501.0659278E-931.3356631
GCAGAGT30250.030.7627629
ATAGACG1701.2187229E-1030.4109028
CATGGGG13100.030.1365454
TAATAGA2200.029.9082416
ATGGGAG8200.029.8040335
TAGACGA1802.382876E-1028.7214079
Produced by FastQC (version 0.11.2)