FastQCFastQC Report
Sun 14 Apr 2019
SRR6575979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486800
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10080.2070665571076417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.16166803615447822No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7010.14400164338537386No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6610.135784716516023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.074.564371
GTATCAA13750.067.867641
TCAACGC21950.041.5437664
CAACGCA22450.041.4560135
ATCAACG22150.041.3808633
TATCAAC22550.040.855282
AACGCAG22850.040.7303056
ACGCAGA26400.035.068067
CGCAGAG27150.034.0993278
GTACATG34300.033.5270961
ACATGGG34200.033.1229973
TACATGG35150.032.495232
CATGGGG14600.031.8728584
GCAGAGT29200.031.7053729
CTATACA953.7454785E-529.6874
ATGGGAG8350.028.7092865
CATGGGA20200.028.3888054
AGAGTAC27900.027.4557210-11
ATGGGGG5250.026.8596675
ATTATCC4250.026.543673
Produced by FastQC (version 0.11.2)