FastQCFastQC Report
Sun 14 Apr 2019
SRR6575980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464548
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10200.2195682685104661No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7210.15520462901573143No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7100.1528367359239519No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.15111463185720314No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4870.10483308506333038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.068.549491
GGTATCA3500.059.099191
GTACCGT652.7852057E-643.375826
ATCAACG20900.041.1447683
TCAACGC21100.040.309364
TATCAAC21550.039.9166372
CAACGCA21600.039.3762745
AACGCAG21900.038.8368766
CCTTTAT1600.038.1962051
ACCTAAG3500.037.6085741
GGTATAG2200.034.171217
GTACATG33500.033.3986551
TATTGTG1554.1836756E-1133.3480765
CTAAGAC4100.033.2371643
ACGCAGA25800.032.962647
AGGTATA2150.032.784056
GGTAATC1606.002665E-1132.3024758
AAGGGTA1755.456968E-1232.222045
CGCAGAG26600.032.1479158
ACATGGG34200.032.0139773
Produced by FastQC (version 0.11.2)