FastQCFastQC Report
Sun 14 Apr 2019
SRR6575980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464548
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9810.2111730111850659No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.1707035656164702No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6690.14401095258186453No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5950.12808148996443855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.068.403691
GGTATCA3800.059.429551
TCAACGC21950.043.4601974
CAACGCA22350.042.682395
AACGCAG23350.041.2569546
ATCAACG23150.041.0044143
TATCAAC23500.040.7936482
ACGCAGA26900.035.462887
CGCAGAG28050.034.19498
GCAGAGT29450.032.5693369
GTACATG33700.031.2725221
ATGGGAG7500.030.7018855
ACATGGG34300.030.0040743
CTAAGAC3800.029.6795863
GATATAA804.7478467E-429.4052471
GTTAGAG804.7809066E-429.3704243
TACATGG35600.029.3044222
AGAGTAC28750.028.04725310-11
ACCTAAG3700.027.9747221
CATGGGA21850.027.959034
Produced by FastQC (version 0.11.2)