FastQCFastQC Report
Sun 14 Apr 2019
SRR6575987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536942
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13030.2426705305228498No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10880.20262896178730663No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9870.18381873647433056No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9850.1834462567651627No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9260.17245810534471134No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC8130.1514130017767282No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7790.14508084672087487No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC7750.1443358873025392No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7720.14377716773878743No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7470.1391211713741894No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6890.12831925980832196No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6850.12757430038998627No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6450.12012470620662938No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC6420.11956598664287764No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6410.11937974678829372No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6310.11751734824245448No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5950.11081271347743332No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5940.1106264736228494No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5860.10913655478617802No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5760.10727415624033881No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA5700.10615671711283528No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5650.10522551783991568No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5520.1028043997303247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.10094200118448547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.053.7845841
CGCAATC608.822481E-539.163143
CAACGCA20600.039.0110475
ATCAACG21100.038.0866133
AACGCAG22400.036.7154436
CCTTTAT959.3421295E-734.6769031
ACGCAGA23250.034.5614057
CGCAGAG23800.033.38968
TATCAAC25000.032.8970382
CTATTGA5150.032.8697789
TCAACGC25350.031.7012834
GCAGAGT25550.031.654769
GTCTTAT1052.0417083E-631.3743381
TAACAGT1501.0677468E-931.3305074
CATGGGT4250.030.9619144
ATGGGTG1859.094947E-1230.4837365
GTACATG26900.029.3915751
TACATGG26900.029.3505132
ACATGGG26200.029.237823
GGGCTAT5950.029.2242556
Produced by FastQC (version 0.11.2)