FastQCFastQC Report
Sun 14 Apr 2019
SRR6575989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259584
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20170.16013223413444438No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16290.12932841319038668No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15260.12115111020781465No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13700.10876606879731722No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13130.10424076520502007No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12660.10050937452365226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.063.8723141
GGTATCA6800.060.906111
ATCAACG30950.045.702223
TCAACGC31200.045.3360144
CAACGCA32700.043.2563865
AACGCAG33050.042.9404836
TATCAAC37200.038.9126632
ACGCAGA39900.035.450727
CGCAGAG44100.032.096128
GCAGAGT46500.030.7429339
GTACATG56100.030.7047331
TACATGG58150.029.338652
ACATGGG58850.028.8265173
ACCTAAG7250.028.5628621
TTAGGTA5400.026.9773564
CTAACGC700.00884292926.8530353
TAGGTAT5150.026.4619735
GGTATAG5700.026.3819317
CTTAGGT5600.026.0138783
GTCTTAG7300.025.7883871
Produced by FastQC (version 0.11.2)