FastQCFastQC Report
Sun 14 Apr 2019
SRR6575990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537926
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17050.11086359161624161No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16890.10982322946617717No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15840.10299585285637931No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15700.10208553597507292No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15390.10006983430932309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23850.061.2310031
GGTATCA7750.051.05941
ATCAACG30750.045.5520483
TCAACGC31050.044.959094
CAACGCA32600.043.3981825
AACGCAG33850.041.794236
TATCAAC39700.035.756352
ACGCAGA42700.033.3499377
CGCAGAG46150.030.6541448
GTACATG57300.030.0901621
TACATGG57450.029.699792
CTAAGAC9150.028.7676053
ACATGGG59600.028.707273
GCAGAGT50950.028.0429579
TAAGACA9800.027.3382954
CCCGATA700.00883307426.8595492
ACCTAAG6750.025.8223671
GTCTTAG7550.025.582031
AGAGTAC42750.024.46179210-11
CTATTAA4100.022.9796941
Produced by FastQC (version 0.11.2)