FastQCFastQC Report
Sun 14 Apr 2019
SRR6575992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278906
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6320.2265996428904362No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4010.1437760392390268No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3510.12584885230149226No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3350.12011215248148122No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3060.1097143840577112No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2920.10469477171520154No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2880.10326059676019878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAC405.669924E-446.9989243
GTATAAG959.4050847E-734.6369971
GTACTAG550.002711417234.1871641
TATAAGG1151.1305201E-732.7007682
GTATCAA10350.029.9757041
ACATGGG19750.029.0322483
ATAAGGT650.00614697628.9224173
ATAGTCC650.00614697628.9224173
GTACATG20200.028.8559971
TACATGG20450.028.273372
CGACGGT1354.6144487E-727.8512137
CATGGGT4300.027.3249574
ATGGGTA3100.027.2896985
CATGGGG8100.027.270984
TACGACG1406.3405605E-726.8565275
ACATAAC700.008825744526.8565278
ACGACGG1501.1574884E-625.0660936
ACGGTAT1501.1574884E-625.0660939
AACGCAG12850.024.5052766
GACGGTA1551.5400728E-624.257518
Produced by FastQC (version 0.11.2)