FastQCFastQC Report
Sun 14 Apr 2019
SRR6575992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278906
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7530.2699834352792697No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4670.16743992599657231No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4650.16672283851907094No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4050.14521021419402952No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3650.13086846464400193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.12620739604024295No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3230.11580962761647293No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3140.11258273396771673No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3070.1100729277964619No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3000.10756312162520706No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2990.10720457788645638No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2860.10254350928269738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAT550.002666884334.302791
ACATGGG18350.033.5631833
GTACATG18700.032.7894331
TACATGG18850.032.1740882
CGCACTT300.004160366531.3314812-13
ATGGGAT1551.5043042E-930.3316635
CATGGGG7450.030.2909494
CTTACCC804.7633468E-429.3837993
CATGGGT4550.028.9317424
ATGGGTA2600.028.931745
GTATCAA9400.028.6008631
GCCTAAC700.008819587526.860377
TTCGGTT350.00883474126.85555562-63
GCGCTAC350.00883474126.85555512-13
ATGGGAG2750.025.6440415
TAGCGCG1551.5357546E-624.265334
CGCGCGT1551.5381556E-624.2609797
ATGGGGC2552.1827873E-1123.967965
CATGGGA9200.023.5070384
ATGGGGA4150.022.6573895
Produced by FastQC (version 0.11.2)