FastQCFastQC Report
Sun 14 Apr 2019
SRR6575994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411772
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18310.12969516324165659No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16670.11807855659412426No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16570.11737022692049424No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16460.11659106427950121No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16410.1162368994426862No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16220.11489107306278917No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15620.11064109502100906No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14490.10263696970898985No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14460.10242447080690083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.063.083121
GGTATCA7450.049.8443531
TCAACGC30950.045.851444
ATCAACG31600.045.20573
CAACGCA31550.044.977875
AACGCAG32800.043.5502936
TATCAAC38850.036.661662
ACGCAGA39350.036.181727
CGCAGAG43900.032.5387158
GCAGAGT47650.030.0755029
GTACATG55600.026.8842221
GTCTTAG6400.026.440381
TACATGG56100.026.059022
AGAGTAC41250.025.28744710-11
ACATGGG57400.024.9686263
ATAGTAC1151.3793897E-424.516593
TTAGGTA3100.022.7371644
TAGGTAT3200.022.0258465
TAACACT2355.2677933E-921.9953824
CATGGGT13950.021.5581974
Produced by FastQC (version 0.11.2)