FastQCFastQC Report
Sun 14 Apr 2019
SRR6575994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411772
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18460.13075765775210163No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16520.11701606208367923No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15580.11035776315155704No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15440.10936610160847501No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15320.108516106000119No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15040.10653278291395493No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14910.10561195433823592No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14440.10228280487217484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23600.059.073351
GGTATCA7100.057.0495221
TCAACGC30200.045.591834
ATCAACG30300.045.131183
CAACGCA31200.043.9799355
AACGCAG32450.042.2857936
TATCAAC40050.034.3800772
ACGCAGA42000.032.670817
CGCAGAG43850.031.2062858
GCAGAGT47450.029.036899
GTCTTAG7100.028.5247611
GTACATG53000.027.815081
TACATGG54550.026.8782862
ACATGGG54750.026.2641663
AGAGTAC40700.025.07171210-11
TAGGTAT3800.024.7327695
GTATAAG2301.4915713E-1024.5734021
AGGTATA3950.022.6038726
GGTACCT9050.021.823258
TCTTAGG8450.021.6895052
Produced by FastQC (version 0.11.2)