FastQCFastQC Report
Sun 14 Apr 2019
SRR6575995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953147
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13510.14174099063418338No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12420.1303051890212108No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12210.12810196118751882No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12050.1264233114094678No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10570.11089580096249581No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10420.10932206679557298No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10080.10575493601721454No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9610.10082390229418967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.062.4775351
GGTATCA5000.054.5330851
ATCAACG22150.042.0126083
TCAACGC22350.041.6366544
CAACGCA22800.040.8148775
AACGCAG23700.039.4632536
ACGCAGA27850.033.7514887
TATCAAC29600.032.2408452
ATACGGT600.00416108231.3326346
CGCAGAG29800.031.0697778
GTACATG36200.030.648241
TACATGG37200.029.9507352
ACATGGG37750.029.1331253
GCAGAGT31900.029.024439
AGAGTAC27250.027.16453210-11
GTCTTAG3200.026.4438441
GGTACCT6350.024.4246528
ATGGGTA7650.023.9602495
GTATAGG2002.291381E-823.5056421
CATGGGG23000.022.4777584
Produced by FastQC (version 0.11.2)