FastQCFastQC Report
Sun 14 Apr 2019
SRR6575995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953147
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12300.12904620168767253No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11510.12075786840854558No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10500.1101613916845985No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10200.10701392335075283No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10190.10690900773962463No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9870.10355170818352258No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG9720.10197797401659975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.062.620871
GGTATCA4850.048.586321
ATCAACG19750.044.9844443
TCAACGC20050.044.7802664
CAACGCA20550.043.9171645
AACGCAG21350.042.2715576
TATCAAC25700.035.118492
ACGCAGA27050.033.1850627
GTACATG35550.031.6842921
CGCAGAG28750.030.8958728
ACATGGG36300.030.8203813
TACATGG37300.029.8680732
GCAGAGT31500.028.0494179
TAGGTAT1555.0762537E-827.2932785
CTTAGGT1458.635743E-725.9352043
AGAGTAC26600.025.17733410-11
ATATCCG950.001303599124.7395215
GTCTTAG3300.024.2784371
ACCTAAG2151.8189894E-924.1123711
CATGGGG21650.023.666624
Produced by FastQC (version 0.11.2)