FastQCFastQC Report
Sun 14 Apr 2019
SRR6575996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870019
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11970.13758320220592885No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11620.13356030155663268No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11410.13114656116705498No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10730.12333063990556528No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9810.11275615819884392No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9590.11022747779071491No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9380.1078137374011372No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8970.10310119664053313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13500.064.408161
GGTATCA4150.058.892251
TCAACGC19100.045.0189934
ATCAACG19200.044.7845233
CAACGCA20050.042.651585
AACGCAG20650.041.4123086
ACGCAGA25050.034.3258637
TATCAAC26150.033.2508662
CGCAGAG26950.031.382818
GTACATG34000.030.9650651
GCAGAGT29250.029.2363829
TACATGG35800.029.1455922
ACATGGG36250.028.775513
TATAAGC1005.3216427E-528.2003272
AGAGTAC25200.026.47682410-11
TAATACC1301.08976E-525.3007144
CTTAGGT1501.1647826E-625.0597573
TAGGTAT1551.5497608E-624.2513755
GTCTTAG2853.6379788E-1223.0879881
CTAAGAC4150.022.6443563
Produced by FastQC (version 0.11.2)