FastQCFastQC Report
Sun 14 Apr 2019
SRR6575996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870019
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11510.13229596135256816No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11180.12850294074037463No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9700.11149181799477942No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9690.1113768779762281No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9470.10884819756809909No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9200.10574481706721348No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG9200.10574481706721348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.064.992041
GGTATCA4450.051.809551
ATCAACG19350.045.4134943
TCAACGC19450.045.1800084
CAACGCA20200.043.502535
AACGCAG20600.042.8859376
ACGCAGA25950.033.8632437
TATCAAC26350.033.709742
CGCAGAG27000.032.39658
GTACATG35250.029.6324541
GCAGAGT30050.029.4213269
TACATGG35900.029.324312
ACATGGG36100.028.637813
GTCCTAA1005.2824642E-528.2309191
GTATAGA1205.791484E-627.4467281
TATATAC1659.327596E-825.6320233
AGAGTAC26200.024.85978710-11
CATGGGT8500.023.7724444
AGCTACG701.922579E-523.5149468-69
CATGGGG21200.023.05274
Produced by FastQC (version 0.11.2)