FastQCFastQC Report
Sun 14 Apr 2019
SRR6575997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270854
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15330.12062754651596486No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13190.10378847609560185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18300.061.9224321
GGTATCA6400.055.8361741
TCAACGC24950.044.831514
ATCAACG25300.044.211313
CAACGCA25900.043.1871075
AACGCAG27250.041.0475656
TATCAAC32700.034.941422
GTACTAG550.002711471434.196321
ACGCAGA33650.033.3802647
GTACATG47850.031.1500931
GTCTTAG5200.030.7438051
TACATGG48150.030.4677472
TAGGTAT3000.029.7652345
CGCAGAG38050.029.1497158
ACATGGG50500.028.9431633
GCAGAGT39800.027.7499229
ATGGGAG12550.027.7118115
AGGTATA3200.026.4362286
ACCTAAG4700.026.011031
ATAGTAC1501.1631819E-625.065463
Produced by FastQC (version 0.11.2)