FastQCFastQC Report
Sun 14 Apr 2019
SRR6575998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153385
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13930.1207749363829077No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12600.10924366104986627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.058.2363471
GGTATCA6450.055.38581
TCAACGC21900.043.9849054
ATCAACG22500.042.8119743
CAACGCA22800.042.4547465
AACGCAG24050.040.443546
TATCAAC28850.033.727792
ACGCAGA29250.033.253587
TACCCGA600.00416586531.3258345
GTACATG42700.029.9423271
TACATGG43450.029.3185772
ACATGGG42950.029.1012973
CGCAGAG34150.028.48228
TAGGTAT3800.028.4405575
GTCTTAG5900.027.0876581
AACCGTG2500.026.31377
GCAGAGT36800.026.3023439
TTAGGTA3950.026.1709484
GAACCGT2700.026.104866
GGTATAG3900.025.3016347
Produced by FastQC (version 0.11.2)