FastQCFastQC Report
Sun 14 Apr 2019
SRR6576000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122331
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC21410.1907636873613934No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17050.1519159677492647No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16500.14701545266057875No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14890.13267030849187983No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14880.1325812082175401No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14640.13044280163338623No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14460.12883899669527082No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14450.1287498964209311No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13880.12367118078356563No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT13790.12286927831450793No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13750.12251287721714896No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13250.1180578635001617No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13020.11600855719034758No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12960.11547395554430911No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11970.1066530283846744No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11960.10656392811033465No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11880.10585112591561668No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT11380.10139611219862946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23700.053.159151
CAACGCA32000.039.2048655
ATCAACG32250.038.609563
GTATAAG4900.035.497451
GTACTAG6050.034.966221
AACGCAG36700.034.568176
TCAACGC36850.034.1724364
GGTATCA10800.033.9517671
TGATCGC4250.033.1658869
ACGCAGA39050.031.8848327
TATCAAC40450.031.6112692
CGCAGAG40000.031.127578
GTGATCG4650.030.3129088
GTACATG48650.028.7955231
GCAGAGT43600.028.772939
TACATGG48750.028.350732
TAGGCAT7300.028.3209935
ACATGGG48500.027.99853
TGCGAGT5050.027.91561994
TAAGGTG5350.027.2261714
Produced by FastQC (version 0.11.2)