FastQCFastQC Report
Sun 14 Apr 2019
SRR6576000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122331
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20990.18702147583912412No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT18000.16038049381154046No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14960.13329401041225808No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14850.13231390739452087No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14440.12866079614659134No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13460.11992896926129636No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13280.11832516432318096No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13170.11734506130544377No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13060.11636495828770657No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12840.11440475225223219No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12030.10718763003071285No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11930.10629662728731543No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11880.10585112591561668No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11880.10585112591561668No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11660.10389091988014232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25100.056.084751
ATCAACG32200.042.4688953
CAACGCA32250.042.2592165
AACGCAG36500.037.8536386
GGTATCA10200.037.8495751
TCAACGC36950.037.136614
GTACTAG4550.036.2162931
TATCAAC40150.035.818582
ACGCAGA38450.035.198977
CGCAGAG39950.033.8985068
GCAGAGT43000.031.4940769
TAGGCAT7250.028.5212025
GTACATG45850.026.1847341
TAGTACT7200.026.1072624
TACATGG46200.025.838322
AGAGTAC40350.025.75452410-11
ACATGGG46050.025.4099353
GTATAAG6300.024.6615721
CTAGGCA8600.024.5893974
CTAGTAC8650.024.4472643
Produced by FastQC (version 0.11.2)