FastQCFastQC Report
Sun 14 Apr 2019
SRR6576001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891163
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21140.23721810712518362No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19990.22431362163824126No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15740.17662313179519348No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10760.12074109899086923No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8940.10031834804631701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15800.059.5924841
GGTATCA5250.055.596951
TCAACGC21000.044.0918434
ATCAACG21050.043.9871143
CAACGCA21800.042.6894045
AACGCAG22650.041.294886
TATCAAC24200.038.455742
ACGCAGA28300.032.882577
CGCAGAG30050.031.1240148
GTACATG37000.031.0460761
TACATGG38100.030.1006722
ACATGGG38250.029.859753
CGTATAG804.7338067E-429.4237881
GCAGAGT32350.028.9111759
TTAGGTA4900.026.8579754
CGTACAC1258.019337E-626.3208183
GTCTTAG5750.026.1999661
CTTAGGT5150.025.554193
AGGTATA4800.025.4591246
TAGGTAT5050.025.1294925
Produced by FastQC (version 0.11.2)