FastQCFastQC Report
Sun 14 Apr 2019
SRR6576006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369848
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25650.18724705222769242No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23040.1681938434045237No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18170.13264245376129322No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15800.11534126414025497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.063.765441
GGTATCA7100.051.772571
ATCAACG28450.047.91463
TCAACGC28600.047.4989474
CAACGCA29650.045.6583255
AACGCAG30750.044.025026
TATCAAC35600.038.6874242
ACGCAGA39250.034.7266547
GTCTTAG8700.033.584271
ACCTAAG10200.032.803611
CGCAGAG42850.031.9188168
CTAAGAC11600.031.2021773
GTACATG55200.030.5638051
GCAGAGT45900.029.8980649
TTAGGTA6850.029.5073384
TACATGG55800.029.4839742
ACATGGG57650.028.211683
CCTAAGA12450.027.561682
TAAGACA13150.027.5243534
TAGGTAT6900.027.2497845
Produced by FastQC (version 0.11.2)