FastQCFastQC Report
Sun 14 Apr 2019
SRR6576007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216413
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22280.18316147558436158No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21770.17896882062260105No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17750.14592083445342988No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14880.1223268741784246No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14430.11862747274157708No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12940.10637834353957085No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12810.10530962756892602No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12810.10530962756892602No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12800.10522741864810718No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12700.10440532943991884No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12680.10424091159828117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.059.2021871
TAGTGCG250.005241680456.3848575
GGTATCA7800.051.8129841
ATCAACG28350.041.600823
TCAACGC28550.041.4739764
CAACGCA29000.040.6683855
AACGCAG30200.039.0524256
ACGTCGA250.001704647737.58990568-69
GTCTTAG7850.035.319711
TATCAAC34200.034.9013632
ACGCAGA36100.032.5397387
TAGGTAT6750.031.3249235
CTTAGGT6700.030.8573843
AGGTATA6750.030.6288136
TTAGGTA6950.030.4234834
GTACATG49800.030.007691
CGCAGAG39700.029.7073888
TACATGG50950.029.1459142
ACATGGG52300.028.390083
GCAGAGT43950.027.048489