FastQCFastQC Report
Sun 14 Apr 2019
SRR6576007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216413
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25200.20716648046346103No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21780.17905102954341987No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17810.14641408797834288No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15130.12438209719889545No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13040.10720043274775919No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12600.10358324023173052No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12310.10119918152798432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.060.6644631
GTATCAA21200.057.04611
TCAACGC27700.043.6006054
ATCAACG27650.043.339533
CAACGCA28350.042.435185
AACGCAG29900.040.0781976
GTCTTAG9000.035.031681
TATCAAC35200.034.1786042
TAGGTAT6250.033.835435
ACGCAGA35950.033.3334667
CTTAGGT6550.032.2857133
AGGTATA6600.031.3290986
ACCTAAG9400.030.53731
GTACATG47950.030.422981
TCTTAGG8350.030.3923512
CGCAGAG40150.030.0991768
GGTATAG7250.029.1684727
TACATGG50900.028.6220632
CTAAGAC11250.028.405053
ACATGGG50800.028.214693