FastQCFastQC Report
Sun 14 Apr 2019
SRR6576008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532048
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31110.2030615228765678No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23630.15423798732154606No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22170.14470825979342683No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18200.11879523356970538No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16610.10841696865894541No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15920.10391319332031372No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15640.10208557434231826No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15600.10182448591689033No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15370.10032322747067976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8900.055.6337241
GTATCAA23100.055.321781
TCAACGC31850.039.843654
ATCAACG32250.039.057993
CAACGCA32900.038.1434675
AACGCAG34050.037.1312836
TATCAAC39000.032.4184652
TAGGACA4450.031.6858984
AGGTATA6300.030.5877916
CTTAGGT6150.030.5695933
GTACATG61900.030.1677821
TACATGG62900.029.3661292
ACGCAGA43800.028.8638737
ACATGGG62600.028.8311333
TAGGTAT6700.028.060155
CGCAGAG45400.027.7431268
GTCTTAG8700.027.1012671
TTAGGTA7250.025.9314484
ACCTAAG10550.025.9247381
GGTATAG7500.025.6920687