FastQCFastQC Report
Sun 14 Apr 2019
SRR6576009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25700.18888725562251948No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22810.167646626488314No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21620.15890048508011173No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17250.12678230192562104No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16490.12119653094223137No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16190.11899162134352491No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15670.11516977803910039No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15540.11421431721299427No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15280.11230339556078202No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14420.10598265471115684No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13950.10252829633985007No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13650.10032338674114362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20650.060.7757261
GGTATCA6600.057.6872751
CTAACGC250.00524190456.384613
TCAACGC28000.043.1275144
ATCAACG29000.041.8023833
CAACGCA29400.041.0738225
AACGCAG30600.039.9237446
CTTAGGT6750.035.5014233
GTCTTAG9300.034.8742681
TAGGTAT6650.033.9155585
TATCAAC36150.033.8055532
AGGTATA6800.033.167426
TTAGGTA7100.033.0895584
ACGCAGA38600.031.5276647
CGCAGAG40650.029.706538
GGTATAG7700.029.2907077
ACCTAAG9850.028.6321531
TAAGACA14150.027.8934464
GTACATG55200.027.1638031
CTAAGAC13400.027.0000923
Produced by FastQC (version 0.11.2)